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However, instead of being transported into mitochondria for oxidative metabolism, pyruvate is converted into lactate by LDHA, coupled with NADH/NAD+ interconversion (5)

For metabolic analysis of cultured cancer cells and tumours, frozen tumour tissue was homogenized in 80% methanol containing 20 mM NEM using a Precellys cold tissue homogenizer (Bertin) at a ratio of 20 mg tissue to 1 ml solvent

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