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Prog Neuropsychopharmacol Biol Psychiatry 31(7):14931499

Immunoblotting Proteins were extracted from cultured SCD erythroblasts or spleen Ter119+ erythroblasts with modified RIPA buffer (150 mM NaCl, 50 mM Tris, pH 7.5, 1% NP40, 0.25% Na-deoxycholate) plus protease inhibitor cocktail (Sigma) and stored at 80 C up to processing

The progression of diabetic nephropathy is multifaceted, influenced by factors such as renal hemodynamic abnormalities, dyslipidemia, oxidative stress, and hormone synthesis, including Angiotensin II (Ang II)

Human data lacking To date, most (if not all) data is preclinical (rodents, cell culture)

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