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The acidic extracts were analysed by using positive mode, and gradient eluted by using water and methanol containing 0.1% formic acid, while the basic extracts were analysed by using negative mode and eluted by using water/methanol containing 6.5 mM ammonium bicarbonate

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When a doctor diagnoses lentigo maligna melanoma, this means that the cancer has spread from the skin to other tissue in the body.