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Leutert, M., Rodriguez-Mias, R

A total of twenty microliters was prepared, consisting of 2 L of nanobody (20 M), 0.4 L of 250 mM TCEP, and 1 L of 200SYPRO Orange dye (Sigma-Aldrich) in PBS

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The effectiveness of pulsed electric field extraction depends on various factors, including the electric field strength, treatment time, and the specific properties of the plant tissue, such as cell size, shape, and membrane composition ( Another constraint is related to the physical and chemical characteristics of the extraction matrix