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Most studies are small (n=3060), short-term (412 weeks), and the effects observed are modest and temporary melanin index returns toward baseline after supplementation stops

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In further studies, only the formation of [14C]carbon dioxide from [14C-1]glucose was measured, with the addition of: sodium ascorbate (which undergoes a non-enzymic reaction in air to produce hydrogen peroxide) acetylphenylhydrazine (which is known to precipitate haemolysis in sensitive subjects, and depletes glutathione) methylene blue (which oxidises NADPH) All incubations were repeated in the presence and absence of N -ethylmaleimide, which undergoes a non-enzymic reaction with the -SH group of reduced glutathione, and thus depletes the cell of glutathione

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Ultrastructural and immunolabeling studies have revealed that type B cells display only a small fraction of apical surface and a single basal body