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Blots were incubated in the appropriate primary antibodies (Supplementary Table 1) followed by HRP-conjugated secondary antibody in blocking buffer
Identity for lot IP10-260411 is confirmed by HPLC-UV with coupled LC-MS
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Cell Metabolism, 27 (4), 740756
Best Practices to Minimize Local Irritation (Research Context Only) General laboratory handling considerations may include: Rotating administration sites Avoiding repeated use of the exact same location Allowing alcohol to fully dry before administration Injecting slowly Ensuring complete dissolution before drawing solution Avoiding overly concentrated reconstitution ratios Avoiding overly shallow (intradermal) placement and ensuring material is delivered into the intended subcutaneous layer when applicable Very superficial placement into the dermal layer may increase the likelihood of visible welts or raised bumps, as fluid deposited intradermally tends to remain localized and elevated rather than dispersing within subcutaneous tissue
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