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After 0 h, 24 h, 48 h, 72 h, and 96 h of inoculation, the CCK-8 reaction solution was added according to the instructions of the CCK-8 kit (Beyotime, Shanghai, China), and the absorbance value at the wavelength of 450 nm was detected using an TECAN reader after incubation at 37 C for 1 h

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Furthermore, the differences between the agents in terms of the duration of their effect may be due to tachyphylaxis and/or receptor desensitization

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Burnout & Overstimulation: Drugs and alcohol initially flood the brain with dopamine, but long-term use can damage dopamine receptors, making it harder to feel pleasure naturally