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Instead, they chemically reduce or hydrolyze the reactive intermediate directly, converting a dangerous functional group into a less reactive one

This is achieved because of the nature of FreSHtracer, which can pass through the cell membrane and react with the thiol of GSH in the cell with high efficiency, unlike other fluorescent dyes that have several shortcomings such as irreversibility, slow kinetics [38], and low fluorescent quantum yields [39]

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Studies indicate it stimulates the release of growth hormone with a high degree of specificity, minimizing impact on cortisol or prolactin levels