While the exact mechanisms remain to be agreed upon, both in vitro and in vivo experiments suggested several potential pathways for APOE4, including enhancing A production (promoting APP transcription and processing), facilitating A aggregation (interaction with soluble and fibrillary A aids in seeding/oligomerization/protofibril formation), and impairing A clearance (disrupted glial and enzymatic A degradation functions, and A removal rate from the brain)
The list of differentially expressed genes then underwent Gene Ontology (GO) and pathway enrichment analyses using the Protein ANalysis THrough Evolutionary Relationships (PANTHER) classification system ( [26,27]
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