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MTHFR mutations have also been recorded in patients with major depressive disorder, indicating a connection between the mutation and the disease

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The cells or tissue sections were fixed in 4% paraformaldehyde in PBS for 15 min, and permeabilized with 0.1% Triton X-100 (Sigma-Aldrich) and blocked with 5% goat serum in PBS and then incubated with anti--catenin (1:100

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The latter is referred to as the senesence associated secretory phenotype (SASP) and this is considered to be driving many aspects of functional decline