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Any deviation from this baseline in subsequent vials, particularly those with different storage histories, becomes meaningful

The purpose of this treatment is to eliminate matrix constituents that could interfere with the detection process or damage analytical equipment, and to enrich the analyte in the sample so that it is readily available for detection [3], [2]

Vitamin B12 is an essential nutrient that humans need to ingest on a regular basis because it is a water soluble vitamin that cannot be stored in your body