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Cold solutions can cause incomplete dissolution and increase the risk of the peptide precipitating out of solution
Lipid peroxidation assay and enzyme-linked immunosorbent assay (ELISA) analysis Tissue lipid peroxidation markers, including malondialdehyde (MDA), glutathione (GSH), glutathione peroxidase (GSH-Px), and Fe 2+ contents, were detected following the respective manufacturers protocols
SIRTs play important roles by stimulating antioxidant expression, repairing cells damaged by oxidative stress, and preventing cell dysfunction
M.et al (2021)
The schematics of the experimental setup and NTAPP device are shown in Supplementary Figure 8
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