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Notably, we identified a hypomethylated regulatory region in the promoter of AKT1, corresponding with its overexpression and increased phosphorylation, suggesting that epigenetic modulation may contribute to the observed AKT1 signaling changes

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Immortalized cell lines, such as microglia cell lines BV2 (mouse) and HMC3 (human) and astrocyte cell lines C8-D1A (mouse) and U373 (human), primary microglia and astrocytes isolated from neonatal mouse or rat brain, and human iPSC-derived microglia and astrocytes are all commonly used in cell culture studies

Three types of solvent dispersion methods are currently being used, such as ethanol injection, ether injection, and reverse phase evaporation method

10.27119/d.cnki.ghezc.2022.000124 93 MaT