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The comparative threshold cycle (Ct) method was used to measure the relative expression, where 2 Ct represents the fold change in expression, as previously described (Livak and Schmittgen, 2001)

The recent popularity of peptides as well as increased rates of unregulated use in athletes supports the need for sports medicine provider education on not only the evidence of individual peptides but also the regulatory framework that governs peptide use. Regulations in the pharmaceutical industry are constantly evolving

Neumann, L

The concern is dosing accuracy, not safety

Grant-in-aid for Scientific Research A 18H04015 (H.O.)), and by a grant for Core Research for Evolutional Science and Technology, Japan Science and Technology Agency CREST/JPMJCR14W3 (H.O.)