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Second, you can split the dose across two syringe draws from the same vial

As an alternative strategy to identify the best overlap, we analyzed the relationship between ion transmission and centerness, where the transmission is defined as the relation between the intensity of the extreme m / z compared to the intensity of the centermost precursor for DIA windows in two consecutive scan cycles shifted by half-a-window

Viros A, Sanchez-Laorden B, Pedersen M, Furney SJ, Rae J, Hogan K et al

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The first glimpse of the full-length KAR structure was a relatively low-resolution, 7.6- reconstruction of homomeric GluK2 in the high-affinity agonist (2S, 4R)-4-methylglutamate (SYM 2081)-bound non-conducting state (Meyerson et al., 2014), which was followed by a similar successor structure with improved 3.8- resolution (Meyerson et al., 2016)