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Transmission Electron Microscopy Cells were fixed with 2.5% glutaraldehyde in 0.1 mol/L of Sorensons buffer (0.1 mol/L H2PO4 and 0.1 mol/L HPO4 [pH 7.2]) at 4C overnight, washed three times with PBS, and then treated with 1% OsO4 in 0.1 mol/L of Sorensons buffer for 2 h at room temperature