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To construct the sorting vector pLKO.1-GST DAF for shRNA gene knockdown experiment, we amplified the coding sequence of N-signal-GST-EGFP-GPI DAF from the pEGFP-GST-GPI DAF plasmid and joined it with FastDigest KpnI/FastDigest BamHI (Thermo Fisher) linearized pLKO.1 vector using ClonExpress II One Step Cloning Kit

Single-cell rna sequencing in dissecting microenvironment of age-related macular degeneration: challenges and perspectives

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In 2005 he created Cedar Tree Inc., a nonprofit foundation that works to give people of all walks of life the opportunity to become educated and improve their lives