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The Diagnostic and Statistical Manual of Mental Disorders, 5th Edition, Text Revision (DSM 5-TR) describes OUD as a pattern of opioid use leading to problems or distress, where at least two of the following occurring within a 12-month period: Taking larger amounts or taking drugs over a longer period than intended

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however, the clinical relevance of these effects in adolescents has yet to be established

Wavefront positions are defined as the distance from the TSS where the reads density drops below an arbitrary threshold (dashed line)

gRNA-mediated Cas9 double-strand break of genomic DNA between codons tyrosine-972 and leucine-973 should initiate homologous-directed repair using the ssDNA repair oligo, thereby mutating the PAM sequence, exchanging codon leucine-973 to phenylalanine-973, as well as inserting silent point mutations at codons glycine-962 and proline-963 to generate an ApaI restriction site for genotyping