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Anti-Aging Potential Preliminary research suggests MB may delay signs of aging

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2 Nonantimicrobial sulfonamides include carbonic anhydrase inhibitors, selective cyclooxygenase-2 inhibitors, loop diuretics, sulfonylureas, thiazide diuretics, triptans and other agents

doi: 10.1089/ars.2012.5013

Glutathione concentration was detected after centrifugation of the samples (12,000 g, 20 min, 4 C) using the supernatant, 100 mM phosphate buffer (pH 7.5), 1 mM 5,5-dithiobis(2-nitrobenzoic acid) (DTNB), 1 mM NADPH, 1 U of glutathione reductase in the enzymatic assay