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Here, we performed electrophoresis using pre-cast gels (Novex 420% TrisGlycine Plus Midi Protein Gels, Invitrogen, Thermo Fisher Scientific, Waltham, Massachusetts, USA)

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Measured in a cell proliferation assay using MCF-7 cells

In the randomized placebo-controlled HOME trial of 390 adults with type 2 diabetes (de Jager and colleagues, Long term treatment with metformin in patients with type 2 diabetes and risk of vitamin B-12 deficiency, BMJ, 2010), metformin lowered serum B12 by a mean of 19 percent over 4.3 years