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Nature 537 , 363368 (2016)

doi: 10.1016/j.canep.2024.102642 123 GotardeloDRCourrolLCBelliniMHDe Oliveira SilvaFRSoaresCRJ

After removal of non-adherent cells by washing the plates, monocytes were differentiated into macrophages in RPMI medium (Gibco, Grand Island, NY, USA) supplemented with 10% FCS (Gibco) plus antibiotics and antimycotics (100 U/ml penicillin, 100 g/ml streptomycin, and 25 g/ml amphotericin