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Virus titers were estimated using a qPCR-based method to detect ITR sequence as described previously ( Primary glial cell culture Primary mouse glial cell cultures were prepared from the cortices of postnatal days 25 (P25) pups of C57BL/6 mice as described previously ( 5 astrocytes, immediately after MACS sorting, onto PDL-coated (10 g/ml) 6-well plates

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