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The columns were washed with 15 column volumes of buffer 1 followed by the elution with 200 mM imidazole, 50 mM Na x H y PO 4 , 300 mM NaCl, pH 8.0 at 4 C

The through line of history is that people will try to sell anything with all sorts of fantastical claims, Joshua Sharfstein, a health-policy professor at Johns Hopkins and a former principal deputy commissioner of the F.D.A., told me

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