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Tissue samples were incubated overnight with the primary antibody previously diluted in antibody diluent (Agilent Technologies, S080983-2)

However, as mentioned, researchers exploring GHK-Cu might observe an initial purging phase

Statistical significance for multiple group comparisons was assessed using the nonparametric Kruskal-Wallis rank sum test to detect species with significant differences in abundance, followed by pairwise comparisons using Wilcoxon rank sum tests

Activation of the mitochondrial unfolded protein response does not predict longevity in Caenorhabditis elegans

10.1371/journal.pone.0027706 PLoS One 96 KitamuraK.IwanamiA.NakamuraM.YamaneJ.WatanabeK.SuzukiY.et al (2007)